Plasmid
Part:BBa_I713036:Design
Designed by: Zhenyu Shi Group: iGEM07_Tsinghua (2007-10-26)
pET15b-LacI(-)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 431
Illegal PstI site found at 3987 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3987
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 497
Illegal BamHI site found at 319
Illegal XhoI site found at 324 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 431
Illegal PstI site found at 3987 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 431
Illegal PstI site found at 3987
Illegal NgoMIV site found at 529
Illegal NgoMIV site found at 1147
Illegal NgoMIV site found at 1307
Illegal NgoMIV site found at 1661 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3813
Illegal SapI site found at 2730
Design Notes
The lacI gene is disrupted by digestion with EcoNI and HpaI and then blunting.
Source
pET15b